IDENTIFIKASI SENYAWA METABOLIT SEKUNDER DAN AKTIVITAS TOKSISITAS PADA FRAKSI EKSTRAK KULIT JERUK MANIS (Citrus sinensis)
Abstract
Sweet citrus fruit is a favorite fruit of the people of Indonesia. Data from the Statistics Agency (BPS) recorded that orange peel waste from the production of sweet orange fruit (citrus sinensis) in 2022 in East Kalimantan reached 2,326-2,714 tons/year. Orange peel contains secondary metabolite compounds namely flavonoids, alkaloids, tannins, steroids, pectin, phenolic compounds, saponins and terpenoids. The content of active compounds owned by orange peel has been applied in the world of health, one of which is as an anti-cancer. In this study, the identification of compounds in orange peel extract was carried out by fragmenting concentrated ethanol extract of orange peel by using water solvent as a polar solvent, chloroform as a semi-polar solvent and n-hexane as a non-polar which was then carried out phytochemical tests and toxicity tests using the BSLT method. Based on this study, phytochemical tests show that orange peel extract of water fraction contains phenolic compounds and flavonoids. Chloroform fraction of alkaloid compounds and steroids. n-hexane fraction of alkaloid compounds, steroids, phenolics and flavonoids. The Lc50 value test results in water fraction extract were 381.417 ppm, chloroform fraction extract was 105.438 ppm, and n-hexane fraction extraction was 94.623 ppm. The water fraction and chloroform are classified as moderately toxic while the n-hexane fraction extract is toxic with a group of alkaloid, steroidal, phenolic and flavonoid compounds.
Keywords: : Bagasse, Biocharcoal, Briquettes, Particle Size
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