IDENTIFIKASI SENYAWA METABOLIT SEKUNDER DAN UJI TOKSISITAS HASIL FRAKSINASI EKSTRAK KULIT BUAH NAGA MERAH (Hylocereus polyrhizus)
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Abstract
East Kalimantan, especially the cities of Tana Paser, is one of the producers of dragon fruit, especially red dragon fruit (Hylocereus polyrhizus). The amount of consumer interest in red dragon fruit results in a large amount of dragon fruit peel waste that is not utilized. Several studies have shown that red dragon fruit skin extract has toxic properties that can be applied to cancer cells. So in this study, the extraction and fractionation process was carried out which was then continued with phytochemical tests and BSLT tests with the aim of knowing secondary metabolites in the aqueous extract fraction, ethyl acetate extract fraction and n-hexane extract fraction with phytochemical test methods and to determine the toxicity of the fraction. aqueous extract, ethyl acetate extract fraction and n-hexane extract fraction were carried out using the BSLT test method. The results of the phytochemical test showed that the aqueous fraction extract of dragon fruit peel contained alkaloids, phenolics and saponins. The extract of the ethyl acetate fraction of dragon fruit peel contains phenolics and flavonoids. Fralsi n-hexane dragon fruit peel \contains triterpenoids. The results of the BSLT test showed that the LC50 value of the aqueous fraction extract was 451.855 ppm, the ethyl acetate fraction extract was 374.024 ppm, and the n-hexane fraction extract was 312.176 ppm which overall was classified as moderately toxic. The highest toxicity effectiveness was shown in the n-hexane fraction extract with triterpenoid secondary metabolite compounds.
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